Enhancing saltiness in emulsion based foods
© Lad et al.; licensee BioMed Central Ltd. 2012
Received: 3 January 2012
Accepted: 13 April 2012
Published: 18 July 2012
The concept of enhancing saltiness perception in emulsions and a liquid food formulated with the emulsions (ambient vegetable soup) through increasing salt concentration in the continuous phase while retaining the fat content of the (aqueous continuous) product was evaluated. This was accomplished by increasing the droplet phase volume using duplex emulsion technology. Viscosity and droplet size distribution was measured. Saltiness evaluation was based on simple paired comparison testing (2-Alternate Forced Choice tests, BS ISO 5495:2007).
Single and duplex emulsions and emulsion-based products had comparable mean oil droplet diameters (25 to 30 μm); however, viscosity of the duplex emulsion systems was considerably higher. Sensory assessment of saltiness of emulsion pairs (2AFC) indicated duplex technology enhanced saltiness perception compared to a single emulsion product at the same salt content (6.3 g/100 g) in both simple emulsions and the formulated food product (P = 0.0596 and 0.0004 respectively) although assessors noted the increased viscosity of the duplex systems. The formulated food product also contained pea starch particles which may have aided product mixing with saliva and thus accelerated tastant transport to the taste buds. Lowering salt content in the duplex systems (to levels of aqueous phase salt concentration similar to the level in the single systems) resulted in duplex systems being perceived as less salty than the single system. It appears that the higher viscosity of the duplex systems could not be “overruled” by enhanced mixing through increased droplet phase volume at lowered salt content.
The results showed that salt reduction may be possible despite the added technology of duplex systems increasing the overall measured viscosity of the product. The changes in viscosity behavior impact mouthfeel, which may be exploitable in addition to the contribution towards salt reduction. With a view to applying this technology to real processed foods, it needs to be tested for the product in question but it should be considered as part of a salt reduction tool box.
KeywordsSalt Health Duplex emulsions Microstructure Fat
Salt (NaCl) has been traditionally used as a food preservative to reduce microbial growth, thereby preventing food spoilage. Salt also plays a crucial role in imparting saltiness to foods, and enhancing or masking flavors, as well as being essential for textural attributes of some products . Reported intake of salt by adults in most Western countries has been estimated to be 9 to 12 g per day , which is much greater than the level currently recommended by the World Health Organization (WHO) of a maximum of 6 g per day . The negative health consequences of high salt intake have been widely reported. Of most concern is the direct link to the development of hypertension [4, 5] which subsequently increases the chance of developing cardiovascular disease [6, 7] and renal disease [8, 9].
Excessive intake of salt through the Western diet has been attributed to the relatively large amounts of salt contained in prepared/pre-cooked foods. It has been estimated that over 75% of daily salt intake comes from processed foods . Reduction of the overall amount of salt added to processed food is one possible strategy to reduce salt intake . However, simply reducing the amount of salt added to foods not only represents technological difficulties and, in case of some foods, a safety risk, it inevitably means a compromise in product taste and flavor with negative impact on consumer acceptability. Despite these restraints, overall salt reduction has been successfully applied to staple foodstuffs, such as bread, using gradual step-wise reduction . This approach is time consuming, with levels being reduced slowly over several years. An alternative strategy is to use salts with an alternative cation, as it is the sodium causing the negative health impact. Replacement of NaCl with potassium chloride (KCl) has had mixed results as KCl is often reported to be bitter and can produce a metallic aftertaste [13–15]. The presence of low levels of NaCl [16, 17] and substances imparting umami taste , for example in savory soups [18–20], can mask this bitter taste, rendering low-level substitution of NaCl with KCl feasible. In , it has been reported that naturally brewed soy sauce can replace NaCl without lowering overall taste intensity or decreasing consumer acceptance. Generally, adding components such as arginine , lysine  or glutamate , which enhance the umami character of food, may raise saltiness perception and may be a suitable approach in certain food types. Another method to enhance saltiness perception includes the use of specific odor-taste interactions in the presence of expected flavors [23, 24].
The approach adopted in this study on salt reduction was based on enhancing salt concentration in the continuous product phase and is applicable to aqueous-based foods. Food emulsions, with the droplets acting as inert filler particles excluding salt from the volume corresponding to the dispersed phase volume of the emulsion, were evaluated. This approach has been taken in the past; increase in saltiness perception from oil-in-water emulsions with increasing oil phase volume and constant total amount of salt has been reported [25, 26]. It was also found that with increasing oil phase volume for emulsions of constant continuous phase salt concentration, salt perception declined as the presence of oil obstructed the salt from reaching the taste buds [25, 26]. Recently, a corresponding approach using air bubbles as “tastant excluded fillers” in a water-based gel has been reported . It was described as offering scope for the reduction of sodium chloride and sucrose. In this case, no effect of filler phase volume on taste perception in samples with constant continuous phase tastant concentration was found as air would simply escape leaving the tastant to reach the taste buds. There was, however, an impact on sample texture and appearance due to the presence of 40% volume fraction of air bubbles.
In the literature cited thus far, the salt was dissolved in the aqueous product phase corresponding to the continuous product phase, in which it was equally distributed. Strategies to enhance saltiness perception at equi-salt stimuli by creating localized concentration differences have also been explored. Formulation of products with salt gradients is less of a challenge for low moisture content foods, such as bread, as diffusion is restricted, and it has been successfully applied . The magnitude of saltiness enhancement was shown to depend on the total salt content (highest for lowest level) and sensory contrast (increased with increasing heterogeneity). For liquid foods this approach can only be realized by encapsulating the stimulant and facilitating release during oral processing. Whereas design of suitable food microstructures remains a challenge, the hypothesis of increased taste perception by temporal changes of tastant concentration using pulsed delivery systems has been tested for sweetness  and saltiness perception [20, 30, 31]. With respect to saltiness perception, success has been shown to be mixed depending on the time scale of the experiment. Spiking salt concentration within a delivery period of 15 s had no effect on saltiness perception and overall saltiness perception depended on the overall delivered amount of salt . A 30 s long delivery period on the other hand was shown to increase salt perception based on short and intense stimulus delivery periods .
Here we report the results of a study undertaken to assess salt reduction in a simple ambient vegetable soup. The product was based on emulsion technology and the fat content of the food product was retained constant through application of duplex emulsion technology. Thereby, we specifically refer to oil-in-water emulsions with an included water phase in the internal oil phase. Such duplex emulsions are known as water-in-oil-in-water emulsions, or in short, w/o/w. Based on , samples with higher dispersed phase volume were expected to be perceived as saltier when the overall salt concentration was kept constant, although the emulsion system evaluated in the current study was more complex. Then the hypothesis was tested that reducing salt concentration in the product with the higher dispersed phase volume would lead to no significant difference in perceived saltiness up to a certain level of salt reduction. Salt concentrations used in these studies were chosen to be within the range presently found in commercial soups.
Results and discussion
Microstructure and viscosity
The micrographs of the emulsion samples (Figure 11c) show the typical microstructure of an unflocculated emulsion with a broad droplet size spectrum. The droplets of the duplex emulsion appear dark due to the enclosed water droplets corresponding to the droplet phase of the initially prepared w/o emulsion. The dark appearance of duplex emulsion droplets has previously been reported in the literature [32–34]. The droplet size spectra as well as the d4,3 values of both types of emulsions compare well and, hence, droplet size can be removed as a variable which may influence saltiness perception.
Addition of pea purée to the emulsions to form a simple vegetable soup introduced an additional structure component as can clearly be seen in Figure 1b, 1d in which gelatinized pea starch granules have been highlighted. Their presence is also reflected as an additional peak in the particle size data (Figure 2), which was verified by sizing of the pea purée alone (results not shown).
The increased viscosity of the duplex samples could be expected to affect saltiness perception. Previous studies have shown that viscosity increase leads to a decrease in saltiness perception [36–41]. On the other hand, it has also been demonstrated that including particles in a liquid food may enhance saltiness perception . This is proposed to be due to the solid particles increasing mixing efficiency with saliva and thus salt delivery to the taste buds. Here the two higher viscous samples contain a larger volume fraction of dispersed particles which may promote in-mouth mixing.
Sample pairs presented to assessors
g salt/100 g product
Continuous product phase salt concentration/mol/L
Number of assessors judging this sample to be the saltier of the pair
PC4 and PC5 evaluated whether the overall salt content could be reduced in the duplex emulsion-based product without reducing perception of saltiness when compared to the single emulsion-based product. In PC4, the overall salt concentration of the duplex emulsion-based soup was reduced to 0.57 g salt/100 g compared to 0.63 g salt/100 g for the single emulsion. Paired comparison testing returned insufficient evidence to suggest a significant difference in saltiness between the two samples as shown in Table 1. However, there was a trend in the data pointing towards higher perceived saltiness of the sample in the pair that contained the overall higher salt concentration, which was the single emulsion/soup based product. Indeed, further analysis of the resultant data from PC4 for similarity testing (BS 5495:2007) indicated the sample pair cannot be concluded to be perceptually identical in terms of saltiness. Further salt reduction in the duplex emulsion/soup based product to 0.5 g salt/100 g led to the single emulsion/soup product being perceived as significantly saltier (Table 1). Assessors commented again that the duplex samples were thicker and creamier than the single emulsion samples.
The results of the actual salt reduction tests conducted in this study are complex but to some degree confirm previous findings  that perception of saltiness is a complex function of salt concentration in the aqueous phase and aqueous phase volume. In the present study, the use of single and duplex emulsion systems led to comparison of samples which differed significantly in viscosity. As mentioned previously, studies have shown increasing viscosity commonly decreases tastant perception [36–41], and this may result in a reduced perception of saltiness in duplex emulsions compared to single emulsions irrespective of salt content. It appears that the disparity in viscosity is not “overcome” by the increased dispersed phase volume in the duplex emulsion promoting in-mouth mixing and acceleration of tastant transport to the taste buds. However, this observation depends on the difference in salt content. Thus, to further clarify use of duplex microstructure in salt reduction technology, control of product viscosity and product microstructure would be key to understanding the primary cause of altered taste perception. On the other hand, reducing the fat content of the type of emulsion-based product tested by incorporating water into the droplets of the dispersed phase appeared to have no effect on saltiness perception other than increasing the dispersed phase volume of the emulsion. The study of the viscosity behavior of duplex emulsions has received little attention compared to single emulsions , and it cannot be excluded that the included water droplets affect the viscosity behavior of the duplex system.
This study aimed to provide initial evaluation to assess whether duplex emulsion technology presents an opportunity to reduce salt in emulsion-based liquid or semi-liquid foods while keeping the fat content constant. Emulsions and a more complex emulsion-based ambient vegetable soup were tested using paired comparison tests to identify the sample perceived as overall saltier of each pair. The results showed that salt reduction may be possible despite the added technology increasing the overall measured viscosity of the product. The changes in viscosity behavior impact mouthfeel, which may be exploitable in addition to the contribution towards salt reduction.
To conclude, in view to applying this technology to real processed foods, it needs to be tested for the product in question, but given the interesting novel data presented it should be considered as part of a salt reduction tool box.
All materials used to prepare the emulsions were of food grade quality and used as obtained. Sunflower oil was purchased from a local supermarket, pea protein isolate (PPI) from Myprotein (Manchester, UK), sodium chloride (+99%) from Sigma-Aldrich (Gillingham, UK), potassium chloride (+99%) from Fisher Scientific (Loughborough, UK) and polyglycerol polyricinoleate (Crestamul PR) from AAK Bakery Services (Oldham, UK). The vegetable base was prepared from frozen peas purchased in a local supermarket and bottled water (Evian, Danone, France) was used to prepare the foods.
Emulsions were formed so that the fat content remained constant and comminution conditions were selected to obtain comparable droplet size distributions in single and double emulsions with a mean diameter of 25 to 35 μm. Emulsion interfaces with oil on the inside were stabilized with protein. The internal interface of the w/o/w duplex emulsions was stabilized with polyglycerol polyricinoleate (PGPR). To minimize microstructure instability through diffusion of the internal water phase into the external water phase due to osmotic pressure gradients, potassium chloride was added to the internal water phase. However, “emptying out” was still observed when the samples were kept for longer than one week, probably due to differences in chemical potential between the two aqueous phases as recently demonstrated .
Emulsions and vegetable soup were prepared and analyzed as detailed in the following sections. Preparation and analysis were within the space of 48 h.
A total of 100 g of single emulsion contained 1 g of PPI, 66.36 g of aqueous NaCl solution and 32.64 g of sunflower oil. The molar concentration of the NaCl solution was chosen to obtain the desired salt concentration in the final sample. Batches of emulsion (100 g) were prepared by immersing a high shear overhead mixer (L5M, emulsor screen, Silverson, Chesham, UK) into a glass beaker containing the aqueous emulsion phase, initially mixing for one minute at 3,000 rpm during which the oil phase was added followed by shearing for four minutes at 4,000 rpm. Temperature was not controlled.
Duplex emulsions of the w/o/w type were formed in two stages by initially preparing a w/o emulsion with 30 g 0.2 M aqueous KCl solution/100 g. The oil phase contained 2.86 g PGPR/100 g. One hundred-gram batches of this w/o emulsion were formed by adding both phases to a glass beaker followed by mixing at 7,000 rpm for two minutes. The duplex emulsion was then obtained by emulsifying 48 g w/o emulsion into 52 g aqueous phase containing 1 g/100 g PPI and NaCl at the level required to obtain the desired salt concentration in the final sample. The comminution conditions were the same as in the single emulsion.
Vegetable soups were formulated based on emulsion ingredient and pea puree prepared as described earlier. One hundred grams of soup contained 90 g emulsion (single or duplex) and 10 g pea purée. The two ingredients were mixed manually and it was verified that emulsion microstructure remained intact by assessing the samples microscopically. Pea purée was prepared by initially washing thawed peas with water followed by microwave cooking (800 W, 8 minutes) of 200 g peas in excess water. Once cooked, the water content was adjusted to 25 g water/100 g peas by draining the cooking water and adding fresh water. This composition was then blended using a hand blender until a smooth mixture was obtained, which was then passed through a sieve with fine holes.
The microstructure of the samples was evaluated using a light microscope (Leitz Diaplan, Stuttgart, Germany) applying bright field illumination. Micrographs were taken with a digital camera (Pixalink PLA662, Ottawa, ON, Canada).
Particle size analysis was carried out with a laser diffraction particle size analyzer (Beckman Coulter LS 13230, Miami, FL, USA). All samples were measured in aqueous dispersion and measurement data were analyzed based on the Fraunhofer model using the instrument’s software. Measurements were performed in triplicate and results are shown as volume based density distribution as well as average volume weighted mean diameter d4,3.
Emulsion viscosity was measured in steady shear with a rotational rheometer (MCR301, Anton Paar, Graz, A) fitted with concentric cylinder geometry (C27) at 20°C. Shear rate was stepwise increased from 1 to 500 s-1 and then decreased to 1 s-1. Samples were analyzed in duplicate and both data traces are shown in the Results section. Viscosity values in the Results section are reported as averaged data acquired at 10 s-1 on the increasing and decreasing shear rate ramp.
Sensory assessment of the products was performed using simple paired comparison tests (2-Alternate Forced Choice tests, BS ISO 5495:2007) requiring assessors to determine which of two samples was perceived as saltier. In a first series of paired comparison tests (Table 1), samples (water-based, emulsion-based and complex emulsion/soup-based) with constant salt concentration in the continuous product phase were evaluated. In a second series of paired comparison tests, the overall concentration of salt in the duplex emulsion product was reduced and compared to the single emulsion product (complex emulsion/soup-base). Here, the samples of each pair had a different overall salt content and the concentration in the continuous product phase was increasingly similar. Assessors were recruited by advertisement (soup usage was not a prerequisite), from the students and staff of the University of Nottingham. A total of 41 assessors attended the first session which consisted of tests PC1, PC2 and PC3, and 39 assessors attended the second session of tests PC4 and PC5 (Table 1). Each sample pair was assessed once by each assessor. Sensory evaluation of samples was conducted approximately 24 h after sample preparation. For each pair, samples of 15 mL were presented in a randomized, balanced order across the panel, in plastic containers labeled with a random three-digit code. The order of the three tests was also randomized across the panel.
All testing was carried out in a quiet, air-conditioned room (18°C) and in individual booths with northern hemisphere lighting. A full explanation of the test was provided prior to testing. Assessors were told they would be given a series of pairs of samples. For each pair, assessors were instructed to taste the samples in the order presented and to determine which sample was the highest in “overall saltiness”. “No difference” answers were not allowed. Assessors were informed that other attributes, such as texture, may differ between the samples and they were asked to focus only on the overall saltiness perceived. In addition, space was provided on the test form to give any comments about the samples they wished. Assessors were instructed to cleanse their palate before and between samples with unsalted crackers (99% Fat Free, Rakusen’s, Leeds, UK) and mineral water (Evian).
Data were acquired and results were analyzed as individual two-tailed paired tests (P < 0.05) using Fizz software (Biosystèmes, Couternon, France).
This study was approved by the Ethics Committee in the Medical School of The University of Nottingham.
Sensory assessment of saltiness of emulsion pairs
World Health Organization.
The authors wish to thank the UK Technology Strategy Board for funding this project (TP/6/DAM/6/S/K3004C), the panelists for attending the sessions and the TSB project team, Joanne Hort and Cécile Morris for discussion.
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